ABSTRACT
Objective To explore curative effects of high-dose prednisone used for children with immune thrombocytopenia (ITP).Methods The children with ITP were divided into prednisone group,γ-globulin group and methylprednisolone group.Platelet levels in peripheral blood were recorded after treatments within the 5th day and the 30th day,and the blood pressure levels of children were also recorded during treatments.Thex2 test was used to compare the effective rate of different treatments as well as the incidence rate of hypertension in these 3 groups.Results In new diagnosis children,there was no significant difference in the effective rate among the 3 groups in the 5th day(P ＞ 0.05).The effective rate of prednisone group in the 30th day was significantly higher than γ-globulin group (P ＜0.05),and there was no significant difference than methylprednisolone group (P ＞ 0.05).In persistence and chronic ITP children,the effective rate of prednisone group were both significantly higher than those in γ-globulin group in the 5th days and the 30th day (P ＜0.05),and were also no significantly higher than those in methylprednisolone group (P ＞ 0.05).The incidence rate of hypertension was significantly lower in prednisone group than that in methylprednisolone group (P ＜ 0.05).Conclusion High-dose prednisone oral treatment is safe,effective and worth using in children with ITP.
ABSTRACT
FMS-like tyrosine kinase 3 (FLT3) is a receptor of tyrosine kinase that is constitutively activated in most of acute myeloid leukemia patients and seems to give an adverse prognosis. In order to explore the silencing effect of FLT3 targeted short hairpin RNA (FLT3-shRNA) on acute leukaemia cell line THP-1, three FLT3-shRNAs (shRNA1, shRNA2, shRNA3) were designed and synthesized by transcription system in vitro and then transfected into THP-1 cells. FLT3 mRNA was analyzed by semi-quantitative RT-PCR, FLT3 protein was detected by Flow cytometry and immunofluorescence. The results indicated that FLT3 expression was downregulated by shRNA1 and shRNA3, and shRNA1 showed stronger inhibitory effect. At 48 hours following transfection, the inhibitory rate of 25 nmol/L shRNA1 was 72.95 +/- 2.07%, lasting 72 hours. The 5 nmol/L and more concentration of FLT3 shRNA1 could downregulate FLT3 mRNA level, which displayed a quantity-effect relation; the inhibitory rate of 15 nmol/L shRNA1 was 67.53 +/- 0.66%. FLT3 protein was located on THP-1 cell membrance, its expression was downregulated obviously by shRNA1, at 72 hours following transfection the inhibitory rate of shRNA1 was 79.67 +/- 0.66%. shRNA1 showed the best inhibitory effect on FLT3 protein, the optimal time of which was 72 hours with an inhibitory rate of 79.67%. It is concluded that FLT3-shRNA1 shows a desireable FLT3-targeted inhibitory effect, which can be used for further investigation of FLT3 mechanism or FLT3 targeting treatment.